B1026
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B1026
Sigma
5-Bromo-4-chloro-3-indolyl phosphate disodium salt
for molecular biology, ≥98%, powder
| Synonym: | 5-Bromo-4-chloro-3-indolyl phosphate disodium salt, BCIP®, X-phosphate disodium salt |
| CAS Number: | 102185-33-1 |
| Linear Formula: | C8H4BrClNO4P · 2Na |
| Molecular Weight: | 370.43 |
| MDL number: | MFCD00036757 |
Description
| Substrates | Histochemical substrate for alkaline phosphatase. |
| Application | For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry. BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid. |
| Legal Information | BCIP is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. |
Properties
| grade | for molecular biology |
| assay | ≥98% |
| form | powder |
| solubility | H2O: 20 mg/mL |
| DMF: insoluble | |
| foreign activity | Protease, none detected |
| storage temp. | −20°C |
Safety
| Personal Protective Equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
| WGK Germany | 3 |
References
| reference | McGadey, J., A tetrazolium method for non-specific alkaline phosphatase. Histochemie 23, 180-184, (1970) Abstract |
| Horwitz, J.P., et al. J. Med. Chem. 9, 447, (1966) | |
| Leary, J.J., et al., Rapid and sensitive colorimetric method for visualizing biotin-labeled DNA probes hybridized to DNA or RNA immobilized on nitrocellulose: Bio-blots. Proc. Natl. Acad. Sci. U. S. A. 80, 4045-4049, (1983) | |
| Knecht, D.A., Dimond, R.L., Visualization of antigenic proteins on Western blots. Anal. Biochem. 136, 180, (1984) | |
| Walters, C., et al., Detection of parvovirus B19 in macerated fetal tissue using in situ hybridization. J. Clin. Pathol. 50(9), 749-754, (1997) | |
| Meltzer, J.C., et al., Enhanced immunohistochemical detection of autonomic nerve fibers, cytokines and inducible nitric oxide synthase by light and fluorescent microscopy in rat spleen. J. Histochem. Cytochem. 45(4), 599-610, (1997) |
